A C-13{P-31} REDOR NMR Investigation of the Role of Glutamic Acid Residues in Statherin- Hydroxyapatite Recognition

作者：Ndao Moise; Ash Jason T; Breen Nicholas F; Goobes Gil; Stayton Patrick S; Drobny Gary P^*

来源：Langmuir, 2009, 25(20): 12136-12143.

DOI：10.1021/la901647n

The side chain carboxyl groups of acidic proteins found in the extra-cellular matrix (ECM) of mineralized tissues play a key role in promoting or inhibiting the growth of minerals such as hydroxyapatite (HAP), the principal mineral component of bone and teeth. Among the acidic proteins found in the saliva is statherin, a 43-residue tyrosine-rich peptide that is a potent lubricant in the salivary pellicle and an inhibitor of both HAP crystal nucleation and growth. Three acidic amino acids-D1, E4, and E5-are located in the N-terminal 15 amino acid segment, with a fourth amino acid, E26, located outside the N-terminus. We have utilized C-13{P-31} REDOR NMR to analyze the role played by acidic amino acids in the binding mechanism of statherin to the HA P surface by measuring the distance between the delta-carboxyl C-13 spins of the three glutamic acid side chains of statherin (residues E4, E5, E26) and 31 p spins of the phosphate groups at the HAP surface. C-13{P-31} REDOR studies of glutamic-5-C-13 acid incorporated at positions E4 and E26 indicate a C-13-P-31 distance of more than 6.5 angstrom between the side chain carboxyl C-13 spin of E4 Ind the closes P-31 in the HAP surface. In contrast, the carboxyl C-13 spin at E5 has a much shorter C-13-P-31 internuclear distance of 4.25 +/- 0.09 angstrom, indicating that the carboxyl group ofthis side chain interacts directly with the surface. C-13 T-1p and slow-spinning MAS studies indicate that the motions of the side chains of E4 and E5 are more restricted than that of E26. Together, these results provide further insight into the molecular interactions of statherin with HAP surfaces.

• 出版日期2009-10-20