Antiphytoviral activity of where science meets business bruceine-D from Brucea javanica seeds

作者：Shen Jian Guo; Zhang Zheng Kun; Wu Zu Jian; Ouyang Ming An; Xie Lian Hui; Lin Qi Ying^*

来源：Pest Management Science, 2008, 64(2): 191-196.

DOI：10.1002/ps.1465

• Brucea javanica
• bruceine-D
• tobacco mosaic virus (TMV)
• potato virus Y (PVY)
• cucumber mosaic virus (CMV)
• antiviral activity

BACKGROUND: Brucea javanica (L.) Merr. is widely distributed throughout the southern parts of China and has been used in traditional medicine to treat a variety of diseases. The objective of the present study was to identify the active antiphytoviral compound in the seeds of B. javanica and evaluate the inhibitory activity of the compound against plant virus.
RESULTS: Bioassay-guided fractionation of the most active extract from the seeds led to the isolation of an antiphytoviral compound which was identified as bruceine-D by conventional spectroscopy methods. The compound exhibited significant inhibitory activity against the infection and replication of tobacco mosaic virus (TMV), with IC50 values of 13.98 and 7.13 mg L-1 respectively. The compound also showed a strong inhibitory effect on the infectivity of potato virus Y (PVY) and cucumber mosaic virus (CMV). Furthermore, the compound could effectively inhibit systemic TMV infection in the host tobacco plant under glasshouse conditions.
CONCLUSION: The results suggested that bruceine-D from Brucea javanica may have the potential to be used as a natural viricide, or a lead compound for new viricides.

• 出版日期2008-2
• 单位福建农林大学