Atom force microscopic characterisation of the interaction forces between bovine serum albumin and cross-linked alkylated chitosan membranes in media of different pH

作者：Liu WG; Li F; Zhao XD; De Yao K^*; Liu QG

来源：Polymer International, 2002, 51(12): 1459-1463.

DOI：10.1002/pi.1085

• alkylated chitosan
• protein adsorption
• atomic force microscopy
• adhesion force

Butyl, octyl and hexadecyl moieties were introduced into chitosan. The adhesion of bovine serum albumin (BSA) onto glucose aldehyde-crosslinked alkylated chitosan membranes in pH media was investigated by probing the force-displacement curves with BSA-coated Atom force microscope (AFM) tips. The results indicated that, at the isoelectric point (IP), the sample membranes exhibited higher adhesion forces; and deviating from IP ie at pH 2, pH 6, the adhesion forces decreased. The adhesion forces at pH 2 are less than those at pH 6 due to the presence of electrostatic repulsive and attractive interactions, respectively. Measurements of the adhesion force confirmed quantitatively that the introduction of hydrophobic side-chains to chitosan can facilitate protein adsorption; however, longer flexible side-chains can depress protein adsorption to a certain degree. From an analyses of the adhesion forces, it is proposed that protein adsorption can be tuned by adjusting the lengths of the introduced side-alkyl moieties.

• 出版日期2002-12
• 单位天津大学