科研之友
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摘要
Purpose: To investigate the anti-neuroinflammatory effects of Isodon japonicus (Burm.) Hara extract (IJE) on BV2 microglial cells..
Methods: Cell viability was evaluated by MTT method. BV2 microglial cells were stimulated with lipopolyscarride (LPS, 1 mu g/ml) and the effect of IJE on nitric oxide (NO) levels were measured using Griess assay. Immunoblot analysis was used to assess the effect of IJE on protein expression of inducible NO synthase (iNOS) expression. Tumor necrosis factor-alpha (TNF-alpha) cytokine production was evaluated by enzyme-linked immunosorbent assay (ELISA).
Results: Pretreatment of 100 mg/ml of IJE (p < 0.001) was inhibited nitric oxide (NO) by 1 ug/ml LPS-treated BV-2 cells. iNOS and TNF-alpha expression were attenuated by IJE concentration-dependently (p < 0.001 at 100 mg/ml). IJE scavenged 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radicals in a dosedependently with half-maximal inhibitory concentration (IC50) value of 46.5 mu g/ml.
Conclusion: Data from this study indicate that IJE attenuates neuroinflammatory responses. The strong anti-oxidant effect of IJE modulates expression of inflammatory molecules at the transcription level, and TNF-alpha at post-transcription level.
- 出版日期2018-1
