A PI3 Kinase Inhibitor Found to Activate Bestrophin 3

作者：Qu, Zhiqiang^*; Han, Xiaohua; Cui, Yuanyuan; Li, Chunlin

来源：Journal of Cardiovascular Pharmacology, 2010, 55(1): 110-115.

DOI：10.1097/FJC.0b013e3181c87c85

• anion channel
• chloride channel
• bestrophin 3
• channel activation
• patch clamp
• membrane protein
• channel mutation

Bestrophin 3 (Best3), a member of bestrophin CI channel family, is a CaClcGMP channel candidate in vascular smooth muscle cells. The mechanism for its activation remains unclear. In previous studies, we reported that an autoinhibitory domain ((356)IPSFLGS(362)) existed in Best3 C-terminus and when the autoinhibitory domain was mutated, the Best3 channel was dramatically activated. In this study, we further dissected the roles of the C-terminal sequence in Best3 activation. We found that there were eight basic amino acids downstream of the AI domain within the region (384-397), which were also involved in Best3 activation. Mutations of these basic amino acids significantly activated Best3 as a CI channel. Led by the assumption that the basic amino acids may be involved in the Best3 C-terminal membrane association through binding to membranous phospholipids, we discovered that PI3K alpha inhibitor IV Could strongly activate Best3.

• 出版日期2010-1
• 单位青岛大学