Aqueous and Ethanol Extracts of Australian Cane Toad Skins Suppress Pro-Inflammatory Cytokine Secretion in U937 Cells via NF-B Signaling Pathway

作者：Zulfiker Abu Hasanat Md; Hashimi Saeed M; Qi Ji; Grice I Darren; Wei Ming Q^*

来源：Journal of Cellular Biochemistry, 2016, 117(12): 2769-2780.

DOI：10.1002/jcb.25577

• AQUEOUS EXTRACT
• 60% ETHANOL EXTRACT
• BUFOTENINE
• AUSTRALIAN CANE TOAD SKIN
• U937 CELLS

Toad skin extracts, such as aqueous extracts (AE) of Chinese toad skins, have demonstrated therapeutic benefits for a range of diseases including pain, inflammation, swelling, heart failure, and various types of cancers. In this study, we investigated the anti-inflammatory potential of an AE (0.1-10g/mL) and a 60% ethanol extract (EE; 0.1-10g/mL) from Australian cane toad (Bufo marinus) skins and the known bioactive compound, bufotenine (BT; 0.1-10nM). The assay employed a model of the human monocyte cell line U937 stimulated with lipopolysaccharide (LPS) and phorbol 12-myristate 13-acetate (PMA) for the release of tumor necrosis factor (TNF)- and interleukin (IL)-6. We demonstrated that AE, EE, and BT significantly inhibited the release and expression of TNF- and IL-6 in a dose-dependent manner when the cells were pre-treated at non-cytotoxic concentrations. Further investigation revealed that the inhibition of TNF- and IL-6 release and expression was associated with the suppression of nuclear factor (NF)-kappa ()B activation. These results indicate that AE, EE, and BT are strong inflammation inhibitors, thus have the potential for further development as anti-inflammatory therapeutic agents from a natural source regarded as a feral pest in Australia. J. Cell. Biochem. 117: 2769-2780, 2016.

• 出版日期2016-12