Alterations in Aspergillus brasiliensis (niger) ATCC 9642 membranes associated to metabolism modifications during application of low-intensity electric current

作者：Velasco Alvarez Nancy^*; Gutierrez Rojas Mariano; Gonzalez Ignacio

来源：Bioelectrochemistry, 2017, 118: 1-7.

DOI：10.1016/j.bioelechem2017.06.008

• Membrane permeability
• Electric current
• Lipoperoxidation
• Aspergillus brasiliensis (niger)

The effects of electric current on membranes associated with metabolism modifications in Aspergillus brasiliensis (niger) ATCC 9642 were studied. A 450-mL electrochemical cell with titanium ruthenium-oxide coated electrodes and packed with 15g of perlite, as inert support, was inoculated withA. brasiliensis spores and incubated in a solid inert-substrate culture (12 d; 30 degrees C). Then, 4.5 days after starting the culture, a current of 0.42 mA cm(-2) was applied for 24 h. The application of low-intensity electric current increased the molecular oxygen consumption rate in the mitochondrial respiratory chain, resulting in high concentrations of reactive oxygen species, promoting high lipoperoxidation levels, according to measured malondialdehyde, and consequent alterations in membrane permeability explained the high n-hexadecane (HXD) degradation rates observed here (4.7-fold higher than cultures without current). Finally, cell differentiation and spore production were strongly stimulated. The study contributes to the understanding of the effect of current on the cell membrane and its association with HXD metabolism.

• 出版日期2017-12