Highly Efficient FRET from a Single Nitrogen-Vacancy Center in Nanodiamonds to a Single Organic Molecule

作者：Tisler Julia^*; Reuter Rolf; Laemmle Anke; Jelezko Fedor; Balasubramanian Gopalakrishnan; Hemmer Philip R; Reinhard Friedemann; Wrachtrup Joerg

来源：ACS Nano, 2011, 5(10): 7893-7898.

DOI：10.1021/nn2021259

• nitrogen-vacancy center
• black hole quencher
• DY781
• fluorescence resonance energy transfer
• single molecule
• scanning FRET microscope
• fluorescent nanodiamond

We show highly efficient fluorescence resonance energy transfer (FRET) between negatively charged nitrogen-vacancy (NV) centers in diamond as donor and dye molecules as acceptor, respectively. The energy transfer efficiency is 86% with particles of 20 nm In size. Calculated and experimentally measured energy transfer efficiencies are in excellent agreement. Owing to the small size of the nanocrystals and careful surface preparation, energy transfer between a single nitrogen-vacancy center and a single quencher was identified by the stepwise change of energy transfer efficiencies due to bleaching of single acceptor molecules. Our studies pave the way toward FRET-based scanning probe techniques using single NV donors.

• 出版日期2011-10