New evidence of the involvement of Lichtheimia corymbifera in farmer's lung disease

作者：Bellanger Anne Pauline; Reboux Gabriel; Botterel Francoise; Candido Charline; Roussel Sandrine; Rognon Benedicte; Dalphin Jean Charles; Bretagne Stephane; Millon Laurence^*

来源：Medical Mycology, 2010, 48(7): 981-987.

DOI：10.3109/13693781003713711

• farmer's lung disease
• Lichtheimia corymbifera
• airway cells
• IL-8

Farmer's lung disease (FLD) is a form of hypersensitivity pneumonitis resulting from recurrent exposure to moldy plant materials. We investigated and compared the initial response of respiratory epithelium after exposure to extracts of Sacharopolyspora rectivirgula, Lichtheimia corymbifera (formerly Absidia corymbifera), Eurotium amstelodami and Wallemia sebi. The two criteria for selection of these species were their high prevalence in the hay handled by FLD patients and the presence of high levels of specific precipitins to these molds in FLD patients' sera. Hydrosoluble extracts were prepared from spores and hyphae grown in culture under optimal conditions for each of the four species. Confluent A549 cells were inoculated with one of the four calibrated soluble extracts. Two mediators, one inflammatory (Interleukin (IL)-8) and one allergic (IL-13), were quantified using real-time PCR and ELISA assay, after four exposure periods (30 min, 2 h, 4 h and 8 h). S. rectivirgula and L. corymbifera extracts were the only ones which induced a marked upregulation of IL-8, as shown by both real-time PCR and ELISA assay 8 h after the initial contact. This study adds to the growing body of evidence that L. corymbifera should be recognized as an etiologic agent of FLD along with S. rectivirgula.

• 出版日期2010-11