A mouse model to identify cooperating signaling pathways in cancer

作者：Musteanu Monica; Blaas Leander; Zenz Rainer; Svinka Jasmin; Hoffmann Thomas; Grabner Beatrice; Schramek Daniel; Kantner Hans Peter; Mueller Mathias; Kolbe Thomas; Ruelicke Thomas; Moriggl Richard; Kenner Lukas; Stoiber Dagmar; Penninger Josef Martin; Popper Helmut; Casanova Emilio; Eferl Robert^*

来源：Nature Methods, 2012, 9(9): 897-+.

DOI：10.1038/nmeth.2130

We here establish a mouse cancer model called Multi-Hit that allows for the evaluation of oncogene cooperativities in tumor development. The model is based on the stochastic expression of oncogene combinations (%26apos;hits%26apos;) that are mediated by Cre in a given tissue. Cells with cooperating hits are positively selected and give rise to tumors. We used this approach to evaluate the requirement of Ras downstream effector pathways in tumorigenesis.

• 出版日期2012-9