A more efficient method to generate integration-free human iPS cells

作者：Okita Keisuke^*; Matsumura Yasuko; Sato Yoshiko; Okada Aki; Morizane Asuka; Okamoto Satoshi; Hong Hyenjong; Nakagawa Masato; Tanabe Koji; Tezuka Ken ichi; Shibata Toshiyuki; Kunisada Takahiro; Takahashi Masayo; Takahashi Jun; Saji Hiroh; Yamanaka Shinya

来源：Nature Methods, 2011, 8(5): 409-U52.

DOI：10.1038/NMETH.1591

We report a simple method, using p53 suppression and nontransforming L-Myc, to generate human induced pluripotent stem cells (iPSCSCs) with episomal plasmid vectors. We generated human iPSCSCs from multiple donors, including two putative human leukocyte antigen (HLA)-homozygous donors who match similar to 20% of the Japanese population at major HLA loci; most iPSCSCs are integrated transgene-free. This method may provide iPSCSCs suitable for autologous and allologous stem-cell therapy in the future.

• 出版日期2011-5