ANAEROBIC RESPIRATION OF SHEWANELLA-PUTREFACIENS REQUIRES BOTH CHROMOSOMAL AND PLASMID-BORNE GENES

作者：SAFFARINI DA; DICHRISTINA TJ; BERMUDES D; NEALSON KH

来源：FEMS Microbiology Letters, 1994, 119(3): 271-277.

DOI：10.1016/0378-1097(94)90427-8

• ANAEROBIC RESPIRATION
• SHEWANELLA PUTREFACIENS
• RESPIRATORY MUTANT
• IRON REDUCTION
• MANGANESE REDUCTION

Pleiotropic respiratory mutants, incapable of growth on any electron acceptor other than oxygen, were isolated from two strains of Shewanella putrefaciens (MR-I and sp200). All anaerobic respiratory functions were restored by complementation of the mutants with specific cloned DNA fragments. Southern hybridization experiments revealed that the fragment that complements the MR-1 mutant was localized on the megaplasmids of both strains, while the fragment that complements the sp200 mutant was chromosomal. Neither of these fragments hybridized with the anaerobic regulatory genes of S. putrefaciens (etrA) or E. coli (fnr).

• 出版日期1994-6-15