Listeria monocytogenes Serotype Prevalence and Biodiversity in Diverse Food Products

作者：Hadjilouka Agni^*; Andritsos Nikolaos D; Paramithiotis Spiros; Mataragas Marios; Drosinos Eleftherios H

来源：Journal of Food Protection, 2014, 77(12): 2115-2120.

DOI：10.4315/0362-028X.JFP-14-072

The aim of this study was to assess serotype prevalence and biodiversity of Listeria monocyto genes strains isolated from diverse food products, i.e., minced pork, fruits, and vegetables. Three hundred twenty-six samples previously purchased from supermarkets and street markets within the Athens area were studied for L. monocyto genes prevalence. A total of 121 strains were isolated from the 36 samples that were positive for L. monocyto genes. Serotyping was performed with multiplex PCR, and biodiversity was assessed with random amplified polymorphic DNA (RAPD) PCR analysis using M13, UBC155, and HLWL85 as primers and with repetitive element palindromic (rep) PCR analysis using (GTG)(5) as the primer. The majority (17 of 22) of the contaminated minced pork samples contained strains identified as serotype 1/2a, either alone or in combination with strains belonging to serotypes 1/2b, 4a, 4c, or 4ab. However, all L. monocyto genes isolates from fruits and vegetables belonged to serotype 4b. Rep-PCR provided better differentiation of the isolates than did RAPD PCR and resulted in discrimination of the isolates into a larger number of unique profiles. Complete differentiation was achieved only with the combination of these subtyping techniques.