A micro-chemiluminescence determination of cyanide in whole blood

作者：Lv J; Zhang ZJ^*; Li JD; Luo LR

来源：Forensic Science International, 2005, 148(1): 15-19.

DOI：10.1016/j.forsciint.2004.03.032

• fluidic chip
• chemiluminescence
• cyanide
• whole blood

A reactant volume self-controlled micro-device was presented and applied to the flow injection chemiluminescence (CL) for determination of cyanide in whole blood. A mini distiller was fabricated for cyanide extraction from the blood samples with the extraction efficiencies of cyanide greater than or equal to98%. A fluidic control platform with air driving was fabricated. The described system showed the features of easy fabrication, undiluted sample injection, safe analysis operation, and suitability for automatic cyanide analysis. The calibration curve showed linearity in the cyanide concentration range of 5.0 x 10(-7) to 5.0 x 10(-5) mol l(-1) with the detection limits (3sigma) of 2.3 x 10(-7) mol l(-1). CL peak-height precision was 1.9% R.S.D. (n = 11) at the 1.0 X 10(-6) mol l(-1) cyanide level. The new devices were applied to the analysis of cyanide in rabbit whole blood samples and the results agreed well with those obtained from official method.

• 出版日期2005-2-10
• 单位陕西师范大学