Anti-inflammatory effects and mechanisms of Hizikia fusifonnis via multicellular signaling pathways in lipopolysaccharide-induced RAW 264.7 cells

作者：Sharma Bhesh Raj; Park Chan Seon; Ma Seung Jin; Rhyu Dong Young^*

来源：Pakistan Journal of Pharmaceutical Sciences, 2017, 30(1): 43-48.

• Sub critical water extract
• inflammation
• nuclear factor kappa B
• mitogen-activated protein kinase.

In this study we investigated the anti-inflammatory effects and mechanisms of Hizikia fusifonnis (HF) extracts in lipopolysaccharide (LPS)-induced RAW 264.7 cells. We extracted HF using solvent and sub-critical water techniques. In results, HF extracts inhibited nitric oxide (NO) production in cell-free and LPS-stimulated RAW 264.7 cells. HF210 (extract prepared with sub critical water at 210 degrees C) was most effective. The HF210 extract dose-dependently inhibited inducible nitric oxide synthase expression (iNOS) and nuclear factor kappa (NF-kB) p65 translocation from cytosol to the nucleus. Furthermore, HF210 extract dose-dependently inhibited the phosphorylation of p38 mitogen-activated protein kinase (p38 MAPK), Jun N-terminal kinase (JNK), and signal transducers and activators of transcription (STAT)-1 in LPS-induced RAW 264.7 cells. Thus, our results suggest that anti-inflammatory effects of HF210 extract showed a noticeable distinction by regulation of multiple signaling pathways in LPS-induced RAW 264.7 cells.

• 出版日期2017-1