Analysis of beta(2)-agonists in cattle hair samples using a rapid UHPLC-ESI-MS/MS method

作者：Di Stefano Vita; Pitonzo Rosa; Giaccone Vita; Alongi Angelina; Macaluso Andrea; Cicero Nicola; Cancemi Gabriella; Ferrantelli Vincenzo

来源：Natural Product Research, 2017, 31(4): 482-486.

DOI：10.1080/14786419.2016.1190718

• UHPLC-ESI-MS/MS
• beta(2)-agonists
• cattle hair
• Commission Decision 2002/657/EC

A simple and efficient method was developed for simultaneous analysis of five illegal residual beta(2)-agonists in cattle hair. beta(2)-Agonists were quantified by ultra high performance liquid chromatography coupled with electrospray ionisation tandem mass spectrometry operating in positive multiple-reaction monitoring mode. The method was validated as quantitative confirmatory method according to the EU Decision 2002/657/EC: instrumental linearity, specificity, precision, recovery, decision limit (CC alpha) and detection capability (CC beta) were evaluated. The recovery were greater than 90% and the method appeared suitable for the control of these beta(2)-agonists in cattle hair samples with LOQ values between 4.9 and 5.5 mu g/kg. This method could represent a simple and cheap approach to confirm beta(2)-agonists contamination of cattle for feeding in a not invasive way and before slaughter operations.
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• 出版日期2017