Development of a porcine renal extracellular matrix scaffold as a platform for kidney regeneration

作者：Choi Seock Hwan; Chun So Young; Chae Seon Yeong; Kim Jin Rae; Oh Se Heang; Chung Sung Kwang; Lee Jin Ho; Song Phil Hyun; Choi Gyu Seog; Kim Tae Hwan^*; Kwon Tae Gyun

来源：Journal of Biomedical Materials Research Part A, 2015, 103(4): 1391-1403.

DOI：10.1002/jbm.a.35274

• kidney reconstruction
• extracellular matrix scaffold
• Triton X-100
• sodium dodecyl sulfate
• partial nephrectomy

Acellular scaffolds, possessing an intact three-dimensional extracellular matrix (ECM) architecture and biochemical components, are promising for regeneration of complex organs, such as the kidney. We have successfully developed a porcine renal acellular scaffold and analyzed its physical/biochemical characteristics, biocompatibility, and kidney reconstructive potential. Segmented porcine kidney cortexes were treated with either 1% (v/v) Triton X-100 (Triton) or sodium dodecyl sulfate (SDS). Scanning electron microscopy showed both treatments preserved native tissue architecture, including porosity and composition. Swelling behavior was higher in the Triton-treated compared with the SDS-treated scaffold. Maximum compressive strength was lower in the Triton-treated compared with the SDS-treated scaffold. Attenuated total reflective-infrared spectroscopy showed the presence of amide II (NH) in both scaffolds. Furthermore, richer ECM protein and growth factor contents were observed in the Triton-treated compared with SDS-treated scaffold. Primary human kidney cell adherence, viability, and proliferation were enhanced on the Triton-treated scaffold compared with SDS-treated scaffold. Following murine in vivo implantation, tumorigenecity was absent for both scaffolds after 8 weeks and in the Triton-treated scaffold only, glomeruli-like structure formation and neovascularity were observed. We identified 1% Triton X-100 as a more suitable decellularizing agent for porcine renal ECM scaffolds prior to kidney regeneration.

• 出版日期2015-4