科研之友
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摘要
The objective of this study is to investigate the inhibition effect of anisodamine on idiopathic pulmonary fibrosis (IPF) and endoplasmic reticulum (ER) stress. Fifty healthy male SD rats were randomly divided into 5 groups: the pulmonary fibrosis model group induced by bleomycin (BLM), low concentration of anisodamine group (5 mg/kg), middle concentration of anisodamine group (10 mg/kg), high concentration of anisodamine group (20 mg/kg), and the control group. Histological study was conducted to evaluate the effect of anisodamine on pulmonary fibrosis. Both RT-PCR and Western blot were used to determine the expression of fibrosis related factors alpha-SMA, collagen I and ER stress markers CHOP and GRP78. Histological study showed that anisodamine could significantly inhibit the fibrosis of lung tissues induced by BLM. When treated with BLM, expression of fibrosis related factors alpha-SMA, collagen I and ER stress markers CHOP and GRP78 significantly increased compared with the control. However, anisodamine could inhibit expression of all alpha-SMA, collagen I, CHOP and GRP78 at both mRNA and protein levels under all concentrations, and the effect was in a dose-dependent manner. Anisodamine can inhibit bleomycin-induced pulmonary fibrosis and the effect may be through the inhibition of endoplasmic reticulum stress.
- 出版日期2018
- 单位武汉市中心医院; 华中科技大学
