A filtration probe-free on-line monitoring of glycerol during fermentation by a biosensor device

作者：Sefcovicova Jana; Katrlik Jaroslav^*; Stefuca Vladimir; Mastihuba Vladimir; Vostiar Igor; Greif Gabriel; Bucko Marek; Tkac Jan; Gerneiner Peter

来源：Enzyme and Microbial Technology, 2008, 42(5): 434-439.

DOI：10.1016/j.enzmictec.2008.01.006

• biosensor
• on-line bioprocess monitoring
• probe-free sampling
• glycerol
• 1,3-propanediol
• biodiesel

A novel on-line monitoring approach implementing an improved sampling methodology is presented here. A sample is directly withdrawn from the bioreactor without a need for a filtration or a dialysis probe, extensively diluted in a flow-through fashion and mixed with a mobile phase consisting of a buffer and a soluble mediator. The developed sampling system efficiently controls dilution of the sample prior to biosensor assay covering full dynamic range of glycerol concentrations during its fermentation to 1,3-propanediol. The new sampling protocol minimizes problems associated with the use of traditional on-line sampling methods implementing membrane separation or filtration techniques. Concentration of glycerol in the sample is determined by a flow injection analysis (FIA) with an amperometric biosensor using glycerol dehydrogenase and diaphorase with a throughput of nine samples per hour. The biosensor performance was validated by a reference analytical method (HPLC) with a very good agreement.

• 出版日期2008-4-4