Rifaximin, a pregnane X receptor (PXR) activator regulates apoptosis in a murine model of breast cancer

作者：Gautam Swetlana; Singh Priyanka; Singh Manjari; Roy Subhadeep; Rawat Jitendra K; Yadav Rajnish K; Devi Uma; Gupta Pushpraj S; Saraf Shubhini A; Kaithwas Gaurav^*

来源：RSC Advances, 2018, 8(7): 3512-3521.

DOI：10.1039/c7ra09689e

The present study was proposed to investigate the effect of rifaximin (RFX) on methyl nitrosourea (MNU) induced mammary gland carcinoma in albino wistar rats. Animals were randomized and divided among four groups of six animals each. Group I (control 0.9% normal saline, 3 ml kg(-1), p.o.); Group II (toxic control, MNU 47 mg kg(-1), i.v.); Group III (RFX, 25 mg kg(-1), p.o.); Group IV (RFX, 50 mg kg(-1), p.o.). Toxicity was induced by single i.v. injection of MNU. MNU treatment was evident with increased alveolar bud count, differentiation score, up-regulated inflammatory enzyme markers (COX, LOX, NO and H2S) and oxidative stress markers (TBAR's, protein carbonyl, SOD, catalase and Ach). The mammary gland surface architecture was studied using SEM, carmine staining and H&E staining. The treatment with RFX elicited noticeable restoration of the overall histological architecture in the experimental animals similar to the cntrol. In the MNU treated toxic group, the levels of oxidative stress markers significantly increased in comparison to the control, which was subsequently restored after RFX treatment. Furthermore, RFX up regulated the levels of caspase 3 and caspase 8, when compared to the MNU treated animals. MNU associated toxicity was also ascertained, when determined for UCHL-1, COX, NF-kappa Bp65, BAD, and BCL-xl expression, while RFX demonstrated modulation of the same.

• 出版日期2018