Genetic and functional heterogeneities among fluorescent Pseudomonas isolated from environmental samples

作者：Mehri Ines; Turki Yousra; Chair Mohamed; Cherif Hanene; Hassen Abdennasser; Meyer Jean Marie; Gtari Maher^*

来源：Journal of General and Applied Microbiology, 2011, 57(2): 101-114.

• AHLs
• ARDA
• BOX-PCR
• IAA
• PVD
• Pseudomonas
• TCP

Fluorescent Pseudomonas from diverse environmental samples including wastes were identified and screened for the solubilization of tricalcium phosphate, indole-3-acetic acid (IAA), production and inhibition of extracellular N-acylhomoserine lactone (AHLs) and characterized for their siderophores. Genotypic analysis by amplified rDNA restriction analysis (ARDRA) and BOX-A1R-based repetitive extragenic palindromic-PCR (BOX-PCR) typing resulted respectively in 14 ARDRA types and 24 different BOX-types with diverse incidence among the analyzed strains. Based on 16S rRNA sequence analysis the isolates were assigned to P aeruginosa, P otitidis, P plecoglossicida, P mosselii, P monteilii, P. koreensis, P taiwanenesis, P frederiksbergensis and P graminis. Of the 66 isolates, 56 (84.85%) isolates solubilized tri-calcium phosphate (TCP), 53 (80.30%) isolates produced plant growth hormone IAA, 62 (94%) produced bacteriocin and 34 (52%) isolates produced extracellular N-acylhomoserine lactone while 30 (45%) isolates were able to interfere with N-acylhomoserine lactone. Isolates were clustered into 17 siderotypes and Fe-59 cross-incorporation experiments permitted assignment of all siderotypes but two into well-defined siderovars.

• 出版日期2011-4