Lysobacter spongiae sp nov., isolated from spongin

作者：Choi Heejae; Im Wan Taek^*; Park Jin Sook^*

来源：Journal of Microbiology, 2018, 56(2): 97-103.

DOI：10.1007/s12275-018-7462-3

• Lysobacter spongiae
• 16S rRNA gene
• polyphasic taxonomy

A Gram-negative, motile, aerobic and rod-shaped bacterial strain designated 119BY6-57(T) was isolated from spongin. The taxonomic position of the novel isolate was confirmed using the polyphasic approach. Strain 119BY6-57(T) grew well at 25-30A degrees C on marine agar. On the basis of 16S rRNA gene sequence similarity, strain 119BY6-57(T) belongs to the family Xanthomonadaceae and is related to Lysobacter aestuarii S2-C-T (99.8% sequence similarity), L. maris KMU-14(T) (97.5%), and L. daejeonensis GH1-9(T) (97.3%). Lower sequence similarities (97.0%) were found with all of the other recognized members of the genus Lysobacter. The G + C content of the genomic DNA was 69.9 mol%. The major respiratory quinone was Q-8 and the major fatty acids were C-16:0 iso, C-15:0 iso, summed feature 9 (comprising C-17:1 iso omega 9c and/or C-16:0 10-methyl), summed feature 3 (comprising C-16:1 omega 7c and/or C-16:1 omega 6c), and C-11:0 iso 3-OH. The polar lipids were phosphatidylglycerol, phosphatidylethanolamine, diphosphatidylglycerol, three unidentified phospholipids, and an unidentified polar lipid. DNADNA relatedness values between strain 119BY6-57(T) and its closest phylogenetically neighbors were below 48.0 +/- 2.1%. Based on genotypic and phenotypic characteristics, it is concluded that strain 119BY6-57(T) is a new member within the genus Lysobacter, for which the name Lysobacter spongiae sp. nov. is proposed. The type strain is 119BY6-57(T) (= KACC 19276(T) = LMG 30077(T)).

• 出版日期2018-2