Short Communication: Investigating a Chain of HIV Transmission Events Due to Homosexual Exposure and Blood Transfusion Based on a Next Generation Sequencing Method

作者：Zhao Qi; Zhang Chen; Jiang Yan; Wen Yujie; Pan Pinliang; Li Yang; Zhang Guiyun; Zhang Lei; Qiu Maofeng^*

来源：AIDS Research and Human Retroviruses, 2015, 31(12): 1225-1229.

DOI：10.1089/aid.2015.0178

This study investigates a chain of HIV transmission events due to homosexual exposure and blood transfusion in China. The MiSeq platform, a next generation sequencing (NGS) system, was used to obtain genetic details of the HIV-1 env region (336 base pairs). Evolutionary analysis combined with epidemiologic evidence suggests a transmission chain from patient T3 to T2 through homosexual exposure and subsequently to T1 through blood transfusion. More importantly, a phylogenetic study suggested a likely genetic bottleneck for HIV in homosexual transmission from T3 to T2, while T1 inherited the majority of variants from T2. The result from the MiSeq platform is consistent with findings from the epidemiologic survey. The MiSeq platform is a powerful tool for tracing HIV transmissions and intrapersonal evolution.

• 出版日期2015-12-1
• 单位清华大学; 中国疾病预防控制中心病毒病预防控制所; 中国疾病预防控制中心性病艾滋病预防控制中心