A surface acoustic wave bio-electronic nose for detection of volatile odorant molecules

作者：Di Pietrantonio F^*; Benetti M; Cannata D; Verona E; Palla Papavlu A; Fernandez Pradas J M; Serra P; Staiano M; Varriale A; D'Auria S

来源：Biosensors and Bioelectronics, 2015, 67: 516-523.

DOI：10.1016/j.bios.2014.09.027

• Surface acoustic waves biosensors
• Odorant-binding protein
• Bio-electronic nose
• Laser-induced forward transfer

In this work, a "bio-electronic nose" for vapour phase detection of odorant molecules based on surface acoustic wave (SAW) resonators is presented. The biosensor system is composed of an array of five SAW resonators coated with three types of odorant-binding proteins (OBPs): the wild-type OBP from bovine (wtbOBP), a double-mutant of the OBP from bovine (dmbOBP), and the wild-type OBP from pig (wtpOBP). High resolution deposition of OBPs onto the active area of SAW resonators was implemented through laser-induced forward transfer (LIFT). The resonant frequency shifts of the SAW resonators after the deposition of the biomolecules confirmed the immobilisation of the proteins onto the Al/Au inter-digital transducers (IDTs). In addition, a low increase of insertion losses with a limited degradation of Q-factors is reported.
The "bio-electronic nose" fabricated by LIFT is tested in nitrogen upon exposure to separated concentrations of R-(-)-1-octen-3-ol (octenol) and R-(-)-carvone (carvone) vapours. The "bio-electronic nose" showed low detection limits for the tested compounds (i.e. 0.48 ppm for the detection of octenol, and 0.74 ppm for the detection of carvone). In addition, the bio-sensing system was able to discriminate the octenol molecules from the carvone molecules, making it pertinent for the assessment of food contamination by moulds, or for the evaluation of indoor air quality in buildings.

• 出版日期2015-5-15