A Challenging Study to Identify Target Proteins by a Proteomics Approach and Their Validation by Raising Polyclonal Antibody

作者：Jeong Dawoon; Park Beom Young; Kim Jin Hyoung; Hwang In Ho^*

来源：Korean Journal for Food Science of Animal Resources, 2011, 31(4): 506-512.

DOI：10.5851/kosfa.2011.31.4.506

• Korean native black pig
• landrace
• proteomics
• longissimus dorsi muscle
• polyclonal antibody
• biomarker

This study was conducted to validate the theoretical feasibility of a technique to identify biomarkers in Korean native black pig (KNP) and a commercial Landrace breed. Using two-dimensional electrophoresis, we found six proteins (NADH dehydrogenase Fe-S protein 1, an unnamed protein product, similar to T-complex protein I, annexin V = CaBP33 isoform, fatty acid-binding protein, and catechol O-methyltransferase), which appeared in KNP alone. We raised polyclonal antibodies (used as the primary antibody) for Western blotting to confirm the characteristics of the six KNP proteins. As a result, catechol O-methyltransferase, annexin V = CaBP33 isoform, and the unnamed protein product presented thicker bands in KNP than those in Landrace. Moreover, catechol O-methyltransferase was shown to be more feasible as a biomarker for KNP. However, cross-reactivity was observed with the polyclonal antibodies for KNP and the other three proteins (NADH dehydrogenase, a protein similar to T-complex protein 1, and fatty acid-binding protein). This study only showed limited results from a limited number of animals; however, our research suggests possibilities for future studies.

• 出版日期2011-8