AHNAK1 and AHNAK2 are costameric proteins: AHNAK1 affects transverse skeletal muscle fiber stiffness

作者：Marg Andreas^*; Haase Hannelore; Neumann Tanja; Kouno Michiyoshi; Morano Ingo

来源：Biochemical and Biophysical Research Communications, 2010, 401(1): 143-148.

DOI：10.1016/j.bbrc.2010.09.030

• AHNAK1
• AHNAK2
• Costamer
• Fiber stiffness
• Membrane repair
• Skeletal muscle

The AHNAK scaffold PDZ-protein family is implicated in various cellular processes including membrane repair; however, AHNAK function and subcellular localization in skeletal muscle are unclear. We used specific AHNAK1 and AHNAK2 antibodies to analyzed the detailed localization of both proteins in mouse skeletal muscle. Co-localization of AHNAK1 and AHNAK2 with vinculin clearly demonstrates that both proteins are components of the costameric network. In contrast, no AHNAK expression was detected in the T-tubule system. A laser wounding assay with AHNAK1-deficient fibers suggests that AHNAK1 is not involved in membrane repair. Using atomic force microscopy (AFM), we observed a significantly higher transverse stiffness of AHNAK1(-/-) fibers. These findings suggest novel functions of AHNAK proteins in skeletal muscle.

• 出版日期2010-10-8