科研之友
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摘要
The effect of granulocyte colony-stimulating factor (G-CSF) on post-infarct ventricular remodeling remains controversial. We hypothesized that the timing of G-CSF administration after myocardial ischemia plays an important role in determining its efficacy. Rat myocardial ischemia was induced by 60 min coronary ligation and reperfusion. Surviving animals received G-CSF after 1 h (E-G) or 24 h (D-G) of reperfusion randomly at 100 mu g/kg/d for five consecutive days. 7 days or 3 months post-ischemia, rat hearts were quickly removed for ex vivo electrophysiological measurements or histological analysis (collagen disposition and angiogenesis) and metalloproteinase-2 and -9 activity assays (gelatin zymography). Left ventricular (LV) invasive hemodynamic analysis was performed in 3-month recovery animals before sacrifice. At 3 months post ischemia, LV mechanical remodeling was further impaired with early G-CSF administration (0.65 +/- 0.17%, 13.21 +/- 7.36 mmHg, -4,684 +/- 1,560 mmHg/s) compared with the control group (0.28 +/- 0.12%, 6.45 +/- 3.43 mmHg, -6,267 +/- 1,111 mmHg/s) and D-G group (0.34 +/- 0.12%, 7.90 +/- 5.33 mmHg, -6,227 +/- 1,075 mmHg/s) as shown by increased expansion index (P < 0.01), deterioration of myocardial function with increased LVDP (P < 0.05), and decreased -dP/dt (max) (P < 0.05). By contrast, there was a significant increase in electrical properties including monophasic action potential (MAP) 90 dispersion (12.58 +/- 4.46 vs. 30.56 +/- 6.17 ms at 7 days; 18.54 +/- 4.31 vs. 34.78 +/- 5.24 ms at 3 months; P < 0.05 for both) and inducibility of ventricular arrhythmias (4.78 +/- 1.19 vs. 11.58 +/- 2.76 ms at 3 months; P < 0.05) with early G-CSF treatment compared with the control group. Both early and delayed administrations of G-CSF can improve electrophysiological properties after myocardial ischemia, but have no beneficial effects on LV mechanical remodeling.
- 出版日期2011-6
- 单位天津医科大学; 中国人民武装警察部队后勤学院
