A model for Ca2+ waves in networks of glial cells incorporating both intercellular and extracellular communication pathways

作者：Edwards James R^*; Gibson William G

来源：Journal of Theoretical Biology, 2010, 263(1): 45-58.

DOI：10.1016/j.jtbi.2009.12.002

• Glia
• Astrocytes
• Calcium
• ATP
• Gap junctions
• Purinergic transmission

Networks of glial cells, and in particular astrocytes, are capable of sustaining calcium (Ca2+) waves both in vivo and in vitro. Experimentally, it has been shown that there are two separate modes of communication: the first by the passage of an agent (inositol 1,4,5-triphosphate, IP3) through gap junctions (GJs) joining cells; the second by the diffusion of an extra cellular agent (adenosine triphosphate, ATP) that binds to receptor son the cells. In both cases, the outcome is the release of Ca2+ from internals to resin the glial cells. These two modes of communication are not mutually exclusive, but probably work in conjunction in many cases. We present a model of a two-dimensional network of glial cells that incorporates regenerative intercellular (GJ) and extracellular (ATP) pathways. In the extreme cases of only one type of pathway, the results are in agreement with previous models. Adding an extracellular pathway to the GJ model increased the extent and duration of the Ca2+ wave, but did not significantly change the speed of propagation. Conversely, adding GJs to the extracellular model did increase the wave speed. The model was modified to apply to the retina by extending it to include both astrocytes and Muller cells, with GJs the dominant coupling between astrocytes and ATP responsible for most of the remaining communication. It was found that both pathways are necessary to account for experimental results.

• 出版日期2010-3-7