A Label-free and Separation-free Detection for Melamine Based on Surface Enhanced Raman Scattering

作者：Qu Gan; Zhang Guan Nan; Su Yan; Xu Hui Chun; Zhou Xiao Dong; Shen Ai Guo^*; Hu Ji Ming

来源：Chinese Journal of Analytical Chemistry, 2014, 42(7): 1022-1027.

• Surface enhanced Raman scattering
• Melamine
• Gold nanoparticles

A rapid, sensitive, label-free and separation-free analytical method for determination of melamine (MA) was developed based on surface enhanced Raman scattering (SERS) effect of gold nanoparticles. Through tri-sodium citrate reduction method, gold nanoparticles with average diameter of 30 nm were obtained. The melamine detection platform was constructed after self-assembling 4-mercapto phenylboronic acid (4-MPBA) on the surface of gold nanoparticles through Au-S covalent bond. When MA existed in solution, 4-MPBA functionalized gold nanoparticles would aggregate because of strong hydrogen bond interaction between MA and 4-MPBA. Moreover, following increase of the concentration of MA, gold nanoparticles would aggregate more intensively and form more "hot spots". As a result, Raman signal of 4-MPBA and MA was enhanced greatly. The characteristic Raman peaks of 4-MPBA and MA respectively located at 1076 cm(-1) and 715 cm(-1). Hence, the qualitative and quantitative detection for MA were realized based on the ratio value of I715 cm(-1) to I1076 cm(-1). The linear range of MA detection was 0. 1 mu mol/L-1. 5 mu mol/L. The limit of detection (LOD) reached 0. 02 mu mol/ L in terms of three times signal to noise.

• 出版日期2014-7
• 单位武汉大学