Avidity of influenza virus: Model-based identification of adsorption kinetics from surface plasmon resonance experiments

作者：Wang Wenjing; Wolff Michael W; Reichl Udo; Sundmacher Kai^*

来源：Journal of Chromatography A, 2014, 1326: 125-129.

DOI：10.1016/j.chroma.2013.12.009

• Adsorption kinetics
• Adsorption model
• Multi-site binding
• Influenza virus
• Affinity chromatography
• Surface plasmon resonance

Affinity chromatography and membrane adsorption are highly promising methods for the downstream processing of cell culture-derived influenza virus. For the optimization of this separation process, it is desirable to quantify the kinetics of virus adsorption. For this reason, the adsorption kinetics of the influenza A virus (Puerto Rico/8/34 (H1N1)) on a surface with the immobilized ligand Euronymus europaeus lectin (EEL) was investigated. The adsorption kinetics was experimentally monitored in a microfluidic flow cell by surface plasmon resonance (SPR) spectroscopy. The boundary layer theory was applied to analyze the convective and diffusive mass transport of the virus particles in the SPR flow cell. A multi-site kinetic adsorption model was found to describe the experimentally recorded adsorption curves adequately. According to the proposed model, under the applied experimental conditions, the number of sites (galactose residuals) binding one single virus particle to the EEL surface is in the range of 300 to 460, which is in average about 4% of the total number of sites available on the virus surface. The avidity of individual virus particles to the EEL surface was estimated to be in the order of magnitude of 10(6) M-1 s(-1).

• 出版日期2014-1-24