Molecular detection of tick-borne protozoan parasites in a population of domestic cats in midwestern Brazil

作者：Braga Isis Assis; de Souza Ramos Dirceu Guilherme; Marcili Arlei; Tome Melo Andreia Lima; Goncalves Granjeiro Taques Isis Indaiara; Amude Alexandre Mendes; Chitarra Cristiane Silva; Nakazato Luciano; Dutra Valeria; Pacheco Richard de Campos; Aguiar Daniel Moura^*

来源：Ticks and Tick-Borne Diseases, 2016, 7(5): 1004-1009.

DOI：10.1016/j.ttbdis.2016.05.007

• Feline hemoparasites
• Hepatozoon spp.
• Cytauxzoon spp.
• Babesia spp.

Some tick -borne pathogens that infect domestic cats have been considered emergent in veterinary medicine. Occurrences of Hepatozoon spp., Babesia spp. and Cytauxzoon spp. have been described in several regions of Brazil. This paper offers a comprehensive analysis of the 18S rRNA gene of a Hepatozoon sp. strain detected in domestic cats in the metropolitan area of Cuiaba, in Midwestern Brazil. Based on a molecular analysis, we detected the presence of Hepatozoon species circulating among cats in this region. The aforementioned strain is closely related to other isolates of H. fells detected in wild felids. Moreover, a phylogenetic analysis indicates that this genotype is grouped into a Glade of 18S rRNA sequences previously described for the genus Hepatozoon in wild felids around the world. Hepatozoon fells strains detected in cats from Spain and Israel showed, respectively, 98% and 97% identity to our sequence and are clustered on a separate branch of the phylogenetic tree. This finding suggests a high diversity of Hepatozoon genotypes occurring in cats in Europe and South America. None of the analyzed cats were positive for Babesia spp. or Cytauxzoon spp. by PCR analysis.

• 出版日期2016