Diversity of Plasmids and Antimicrobial Resistance Genes in Multidrug-Resistant Escherichia coli Isolated from Healthy Companion Animals

作者：Jackson C R; Davis J A; Frye J G; Barrett J B; Hiott L M

来源：Zoonoses and Public Health, 2015, 62(6): 479-488.

DOI：10.1111/zph.12178

<jats:title>Summary</jats:title><jats:p>The presence and transfer of antimicrobial resistance genes from commensal bacteria in companion animals to more pathogenic bacteria may contribute to dissemination of antimicrobial resistance. The purpose of this study was to determine antimicrobial resistance gene content and the presence of genetic elements in antimicrobial resistant <jats:italic>Escherichia coli</jats:italic> from healthy companion animals. In our previous study, from May to August, 2007, healthy companion animals (155 dogs and 121 cats) from three veterinary clinics in the Athens, <jats:styled-content style="fixed-case">GA</jats:styled-content>,<jats:styled-content style="fixed-case"> USA</jats:styled-content> area were sampled and multidrug‐resistant <jats:italic>E. coli</jats:italic> (<jats:italic>n</jats:italic> = 36; <jats:styled-content style="fixed-case">MDR</jats:styled-content>, resistance to ≥2 antimicrobial classes) were obtained. Of the 25 different plasmid replicon types tested by <jats:styled-content style="fixed-case">PCR</jats:styled-content>, at least one plasmid replicon type was detected in 94% (34/36) of the <jats:styled-content style="fixed-case">MDR </jats:styled-content><jats:italic>E. coli</jats:italic>; four isolates contained as many as five different plasmid replicons. Nine replicon types (<jats:styled-content style="fixed-case">FIA</jats:styled-content>,<jats:styled-content style="fixed-case"> FIB</jats:styled-content>,<jats:styled-content style="fixed-case"> FII</jats:styled-content>, I2, A/C, U, P, I1 and <jats:styled-content style="fixed-case">HI</jats:styled-content>2) were identified with <jats:styled-content style="fixed-case">FIB</jats:styled-content>,<jats:styled-content style="fixed-case"> FII</jats:styled-content>, I2 as the most common pattern. The presence of class I integrons (<jats:italic>int</jats:italic>I) was detected in 61% (22/36) of the isolates with eight isolates containing aminoglycoside‐ and/or trimethoprim‐resistance genes in the variable cassette region of <jats:italic>int</jats:italic>I. Microarray analysis of a subset of the <jats:styled-content style="fixed-case">MDR </jats:styled-content><jats:italic>E. coli</jats:italic> (<jats:italic>n</jats:italic> = 9) identified the presence of genes conferring resistance to aminoglycosides (<jats:italic>aac</jats:italic>,<jats:italic> aad</jats:italic>,<jats:italic> aph</jats:italic> and <jats:italic>strA/B</jats:italic>), β‐lactams (<jats:italic>ampC</jats:italic>,<jats:italic> cmy</jats:italic>,<jats:italic> tem</jats:italic> and <jats:italic>vim</jats:italic>), chloramphenicol (<jats:italic>cat</jats:italic>), sulfonamides (<jats:italic>sulI</jats:italic> and <jats:italic>sul<jats:styled-content style="fixed-case">II</jats:styled-content></jats:italic>), tetracycline [<jats:italic>tet</jats:italic>(A), <jats:italic>tet</jats:italic>(B), <jats:italic>tet</jats:italic>(C), <jats:italic>tet</jats:italic>(D) and regulator, <jats:italic>tetR</jats:italic>] and trimethoprim (<jats:italic>dfrA</jats:italic>). Antimicrobial resistance to eight antimicrobials (ampicillin, cefoxitin, ceftiofur, amoxicillin/clavulanic acid, streptomycin, gentamicin, sulfisoxazole and trimethoprim‐sulfamethoxazole) and five plasmid replicons (<jats:styled-content style="fixed-case">FIA</jats:styled-content>,<jats:styled-content style="fixed-case"> FIB</jats:styled-content>,<jats:styled-content style="fixed-case"> FII</jats:styled-content>, I1 and I2) were transferred via conjugation. The presence of antimicrobial resistance genes, <jats:italic>int</jats:italic>I and transferable plasmid replicons indicate that <jats:italic>E. coli</jats:italic> from companion animals may play an important role in the dissemination of antimicrobial resistance, particularly to human hosts during contact.</jats:p>

• 出版日期2015-9