Alterations of fetal brain biogenic amine metabolites and monoamine oxidase activity after ethanol treatment

作者：Mao Jian^*; Su Yang; Chen Xuechai; Dou Kaifei; Deng Yulin

来源：Chemistry, 2010, 73(8): 761-764.

• 5-Hydroxytryptamine
• Dopamine
• HPLC
• Monoamine oxidase

Pregnant SD rats were exposed to ethanol to assess whether ethanol treatment could alter monoamine transmitter metabolites and the activity of monoamine oxidase (MAO) in fetal rat brain. The activity of MAO was determined by HPLC-MS with time of flight detector,and the whole brain levels of dopamine (DA), homovanillic acid (HVA), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) were determined by HPLC with electrochemical detector. Ethanol administration inhibited the activity of mitochondrial MAO in fetal brain. The levels of DA and 5-HT in whole fetal brain were not remarkably altered by ethanol treatment, while the levels of HVA and 5HIAA in high dose (4.0g/kg) of ethanol-treated rats were significantly decreased compared to that in the control animals. The reduction of MAO activity and the alteration of DA and 5-HT metabolites may play a role in the central nervous system defects of alcohol teratogenicity.

• 出版日期2010
• 单位北京理工大学