Discovery of a putative acetoin dehydrogenase complex in the hyperthermophilic archaeon Sulfolobus solfataricus

作者：Payne Karl A P; Hough David W; Danson Michael J^*

来源：FEBS Letters, 2010, 584(6): 1231-1234.

DOI：10.1016/j.febslet.2010.02.037

• Acetoin
• 2-Oxoacid dehydrogenase complex
• Archaea
• Thermophile
• Metabolism

Like many other aerobic archaea, the hyperthermophile Sulfolobus solfataricus possesses a gene cluster encoding components of a putative 2-oxoacid dehydrogenase complex. In the current paper, we have cloned and expressed the first two genes of this cluster and demonstrate that the protein products form an alpha(2)beta(2) hetero-tetramer possessing the catalytic activity characteristic of the first component enzyme of an acetoin dehydrogenase multienzyme complex. This represents the first report of an acetoin multienzyme complex in archaea, and contrasts with the branched-chain 2-oxoacid dehydrogenase complex activities characterised in two other archaea, Thermoplasma acidophilum and Haloferax volcanii.

• 出版日期2010-3-19