Assessment of Neuronal Cell-Based Cytotoxicity of Neurotoxins from an Estuarine Nemertean in the Han River Estuary

作者：Kwon Yeo Seon; Min Seul Ki; Yeon Seung Ju; Hwang Jin Ha; Hong Jae Sang; Shin Hwa Sung^*

来源：Journal of Microbiology and Biotechnology, 2017, 27(4): 725-730.

DOI：10.4014/jmb.1611.11027

• Heteronemertean
• Yininemertes pratensis
• tetrodotoxin
• neurotoxicity
• Korean Han River estuary

A heteronemertean, Yininemertes pratensis, was collected in Han River Estuary, South Korea. This estuarine nemertean has been known by the local fishermen for harmful effects to the glass eels, juveniles of Japanese eel Anguilla japonica, migrating to fresh water. The present study confirmed the neurotoxic effects of this heteronemertean ribbon worm at the cellular level. Derivative types of neurotoxic tetrodotoxin (TTX), 5,11-dideoxy TTX (m/z 288) and 11-norTTX-6(S)-01 (m/z 305.97), were identified through HPLC and MALDI-TOF MS. However, significant neurotoxicity was confirmed in the fraction containing an undefined molecule corresponding to the 291.1 (m/z) peak, when tested in rat primary astrocytes and dorsal ganglion cells. This study is the first to report neurotoxins of the estuarine nemertean, fairly abundant in the Han River estuary, and suggests the long-term monitoring of population dynamics and surveillance of the toxicity in this river estuary.

• 出版日期2017-4