Neuregulin-1 Increases Connexin-40 and Connexin-45 Expression in Embryonic Stem Cell-Derived Cardiomyocytes

作者：Wang, Zhi^*; Huang, Jin

来源：Applied Biochemistry and Biotechnology, 2014, 174(2): 483-493.

DOI：10.1007/s12010-014-1089-6

• Neuregulin-1
• Embryonic stem cells
• Cardiomyocytes
• Connexin

Neuregulin-1 (NRG-1) is a vital factor involved in heart development. NRG-1 up-regulated connexin-40 (Cx40) in mice fetal cardiomyocytes has been reported, while the effect of NRG-1 on expression of connexins in embryonic stem cells-derived cardiomyocytes (ESCMs) is limited studied. The process of cardiomyocytes differentiated from embryonic stem cells with or without NRG-1 treatment was observed continuously. Exposure to NRG-1 increased the expression of Cx40 and connexin-45 (Cx45) in ESCMs, while the expression of connexin-43 was unchanged regardless of NRG-1 treatment Western blot analysis also confirmed that the expression of Cx40 and Cx45 in the beating foci was increased in the presence of NRG-1. These results indicate that connexins are differentially regulated by exogenous NRG-1 during cardiomyocytic differentiation of embryonic stem cells.

• 出版日期2014-9
• 单位南京市胸科医院