Agaricus bisporus and Agaricus brasiliensis (1 -%26gt; 6)-beta-D-glucans show immunostimulatory activity on human THP-1 derived macrophages

作者：Smiderle Fhernanda R; Alquini Giovana; Tadra Sfeir Michelle Z; Iacomini Marcello; Wichers Harry J; Van Griensven Leo J L D^*

来源：Carbohydrate Polymers, 2013, 94(1): 91-99.

DOI：10.1016/j.carbpol.2012.12.073

• Agaricus bisporus
• Agaricus brasiliensis (1 -%26gt
• 6)-beta-D-Glucan
• Immunostimulatory activity

The (1 -%26gt; 6)-beta-D-glucans from Agaricus bisporus and Agaricus brasiliensis were purified to evaluate their effects on the innate immune system. THP-1 macrophages were used to investigate the induction of the expression of TNF-alpha, IL1 beta, and COX-2 by RT-PCR. The purification of the polysaccharides gave rise to fractions containing 96-98% of glucose. The samples were analyzed by GC-MS, HPSEC and C-13 NMR, which confirmed the presence of homogeneous (1 -%26gt; 6)-beta-D-glucans. The beta-glucans were incubated with THP-1 derived macrophages, for 3 h and 6 h to evaluate their effects on the expression of pro-inflammatory genes. Both beta-glucans stimulated the expression of such genes as much as the pro-inflammatory control (LPS). When the cells were incubated with LPS + beta-glucan, a significant inhibition of the expression of IL-1 beta and COX-2 was observed for both treatments after 3 h of incubation. By the results, we conclude that the (1 -%26gt; 6)-beta-D-glucans present an immunostimulatory activity when administered to THP-1 derived macrophages.

• 出版日期2013-4-15