microRNA-338-3p functions as a tumor suppressor in human non-small-cell lung carcinoma and targets Ras-related protein 14

作者：Sun, Jiangtao; Feng, Xiaoshang^*; Gao, Shegan; Xiao, Zhongyue

来源：Molecular Medicine Reports, 2015, 11(2): 1400-1406.

DOI：10.3892/mmr.2014.2880

• microRNA-338-3p
• non-small-cell lung carcinoma
• Ras-related protein 14
• cell growth
• cell apoptosis

microRNAs (miRNAs) have been demonstrated to be important gene regulators with critical roles in diverse biological processes, including tumorigenesis. Accumulating evidence suggests that miR-338-3p exerts a tumor suppressor role and is downregulated in tumors, including gastric cancer and colorectal carcinoma. However, the role of miR-338-3p in lung cancer, particularly non-small-cell lung carcinoma (NSCLC), has remained elusive. In the present study, the expression levels of miR-338-3p in NSCLC tissues were compared with those of matched normal tissues by use of polymerase chain reaction analysis. miR-338-3p was shown to be downregulated in NSCLC tissues, and the expression levels of miR-338-3p were significantly correlated with NSCLC cancer differentiation, pathological stage and lymph-node metastasis. Ectopic miR-338-3p expression significantly suppressed the in vitro proliferation and colony formation of NSCLC cells and enhanced apoptosis. Of note, ectopic miR-338-3p expression significantly inhibited Ras-related protein 14 (RAB14) mRNA and protein expression, and reduced luciferase reporter activity containing the RAB14 3'-untranslated region through the first binding site. These findings suggested that miR-338-3p regulated the survival of NSCLC cells partially through the downregulation of RAB14. Therefore, targeting the miR-338-3p/RAB14 interaction may serve as a novel therapeutic application to treat NSCLC patients.

• 出版日期2015-2
• 单位河南科技大学