Deconstruction of the beaten Path-Sidestep interaction network provides insights into neuromuscular system development

作者：Li Hanqing; Watson Ash; Olechwier Agnieszka; Anaya Michael; Sorooshyari Siamak K; Harnett Dermott P; Lee Hyung Kook; Vielmetter Jost; Fares Mario A; Christopher Garcia K; Ozkan Engin; Labrador Juan Pablo; Zinn Kai

来源：eLife, 2017, 6: e28111.

DOI：10.7554/eLife.28111

An 'interactome' screen of all Drosophila cell-surface and secreted proteins containing immunoglobulin superfamily (IgSF) domains discovered a network formed by paralogs of Beaten Path (Beat) and Sidestep (Side), a ligand-receptor pair that is central to motor axon guidance. Here we describe a new method for interactome screening, the Bio-Plex Interactome Assay (BPIA), which allows identification of many interactions in a single sample. Using the BPIA, we 'deorphanized' four more members of the Beat-Side network. We confirmed interactions using surface plasmon resonance. The expression patterns of beat and side genes suggest that Beats are neuronal receptors for Sides expressed on peripheral tissues. side-VI is expressed in muscle fibers targeted by the ISNb nerve, as well as at growth cone choice points and synaptic targets for the ISN and TN nerves. beat-V genes, encoding Side-VI receptors, are expressed in ISNb and ISN motor neurons.

• 出版日期2017-8-15