A simple method of monitoring for cypermethrin resistance in Thrips tabaci (Thysanoptera: Thripidae) using agar-coated glass pipettes

作者：Aizawa Misato^*; Nakai Kiyohiro; Watanabe Takeo; Kumano Akemi; Tamagaki Kumie; Sonoda Shoji

来源：Applied Entomology and Zoology, 2018, 53(1): 165-170.

DOI：10.1007/s13355-017-0541-9

• Insecticide resistance
• Pyrethroid
• Monitoring
• Onion thrips
• Bioassay

A simple plant-free method to monitor for cypermethrin resistance of Thrips tabaci Lindeman at 24 h after insect collection was developed, which utilizes an agar-coated glass pipette. In the laboratory, when cypermethrin-resistant and -susceptible insects were mixed in various ratios and subjected to the method, only resistant insects were detected as survivors. All survivors in the field bioassay were found to have a particular amino acid mutation (T929I) in the sodium channel. Results obtained in this study also showed that the method is affected by temperature, possibly because pyrethroid toxicity increases as the temperature decreases. This method could be used for the on-site monitoring of T. tabaci for cypermethrin resistance with careful temperature management after insect collection.

• 出版日期2018-2