Mitoxantrone and Analogues Bind and Stabilize i-Motif Forming DNA Sequences

作者：Wright Elise P; Day Henry A; Ibrahim Ali M; Kumar Jeethendra; Boswell Leo J E; Huguin Camille; Stevenson Clare E M; Pors Klaus^*; Waller Zoe A E^*

来源：Scientific Reports, 2016, 6(1): 39456.

DOI：10.1038/srep39456

There are hundreds of ligands which can interact with G-quadruplex DNA, yet very few which target i-motif. To appreciate an understanding between the dynamics between these structures and how they can be affected by intervention with small molecule ligands, more i-motif binding compounds are required. Herein we describe how the drug mitoxantrone can bind, induce folding of and stabilise i-motif forming DNA sequences, even at physiological pH. Additionally, mitoxantrone was found to bind i-motif forming sequences preferentially over double helical DNA. We also describe the stabilisation properties of analogues of mitoxantrone. This offers a new family of ligands with potential for use in experiments into the structure and function of i-motif forming DNA sequences.

• 出版日期2016-12-22