科研之友
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摘要
Retinal gliosis is characterized by biochemical and physiological changes that often lead to Muller glia proliferation and hypertrophy and is a feature of many neuro-degenerative and inflammatory diseases such as proliferative vitreoretinopathy (PVR). Although Muller glia are known to release inflammatory factors and cytokines, it is not clear whether cytokine production by these cells mirrors the pattern of factors present in the gliotic retina. Lysates from normal cadaveric retina and gliotic retinal specimens from patients undergoing retinectomy for treatment of PVR, the Muller cell line MIO-M1 and four human Muller glial cell preparations isolated from normal retina were examined for their expression of cytokines and inflammatory factors using semi-quantitative dot blot antibody arrays and quantitative arrays. Comparative analysis of the expression of inflammatory factors showed that in comparison with normal retina, gliotic retina exhibited greater than twofold increase in 24/102 factors examined by semiquantitative arrays, and a significant increase in 19 out of 27 factors assessed by quantitative methods (P<0.05 to P<0.001). It was observed that with the exception of some chemotactic factors, the majority of cytokines and inflammatory factors were produced by Muller glia in vitro and included G-CSF, MCP-1, PDGF-bb, RANTES, VEGF, and TGF2. These results showed that a large number of inflammatory factors expressed by Muller glia in vitro are upregulated in the gliotic retina, suggesting that targeting the production of inflammatory factors by Muller glia may constitute a valid approach to prevent neural damage during retinal gliosis and this merits further investigations.
- 出版日期2016-4
