Aptamer-Based Silver Nanoparticles Used for Intracellular Protein Imaging and Single Nanoparticle Spectral Analysis

作者：Chen, Li Qiang; Xiao, Sai Jin; Peng, Li; Wu, Tong; Ling, Jian; Li, Yuan Fang; Huang, Cheng Zhi^*

来源：Journal of Physical Chemistry B, 2010, 114(10): 3655-3659.

DOI：10.1021/jp9104618

• SCATTERING SUBMICROSCOPIC PARTICLES
• HIGHLY FLUORESCENT ANALOGS
• LIGHT-SCATTERING
• BIOLOGICAL APPLICATIONS
• TRACER LABELS
• TRAFFICKING
• MOLECULES
• DESIGN
• PRPC

Aptamer-adapted silver nanoparticles (Apt-AgNPs) were developed its a novel optical probe for simultaneous intracellular protein imaging and single nanoparticle spectral analysis, wherein AgNPs act as an illuminophore and the aptamer as a biomolecule Specific recognition unit, respectively. It Was found that streptavidin-conjugated and aptamer-functionalized AgNPs show satisfactory biocompatibility and stability in cell Culture medium, and thus not only can act as a high contrast imaging agent for both dark-field light scattering microscope and TEM imaging but also call inspire Supersensitive single nanoparticle spectra for potential intercellular microenvironment analysis. Further investigations showed that caveolae-related endocytosis is likely a necessary pathway for Apt-AgNPs labeled PrPc internalization in human bone marrow neuroblastoma cells (SK-N-SH cells). The integrated capability of Apt-AgNPs to be used as light scattering and TEM imaging agents, along with their potential use for single nanoparticle spectral analysis, makes them a great promise for Future biomedical imaging and disease diagnosis.

• 出版日期2010-3-18
• 单位西南大学