ESBL, plasmidic AmpC, and associated quinolone resistance determinants in coliforms isolated from hospital effluent: first report of qnrB(2), qnrB(9), qnrB(19), and bla(CMY-4) in Algeria

作者：Anssour Lynda; Messai Yamina; Derkaoui Meriem; Alouache Souhila; Estepa Vanesa; Somalo Sergio; Torres Carmen; Bakour Rabah^*

来源：Journal of Chemotherapy, 2014, 26(2): 74-79.

DOI：10.1179/1973947813Y.0000000115

• ESBL
• Hospital effluent
• Plasmidic AmpC
• PMQR determinants

The characterization of extended-spectrum beta-lactamases, plasmidic AmpC (pAmpC), and associated plasmid-mediated quinolone resistance (PMQR) determinants in cefotaxime-resistant coliforms isolated from hospital effluent in Algiers showed bla(CTX-M) genes in 89%, bla(TEM-1) in 79.8%, and pAmpC genes (bla(CIT)) in 2.7% isolates. Association of ISEcp1B with bla(CTX-M) was found in all CTX-M+ isolates, and 97.2% harboured class 1 integrons. Sequencing showed bla(CTX-M-15), bla(CTX- M- 3), and bla(CMY-4) genes. bla(CTX-M-3) and bla(CTX-M-15) were located in Inc L/M conjugative plasmids. The PMQR determinants identified were qnrB1, qnrB2, qnrB9, qnrB19, qnrS2, and aac(6')-Ib-cr. qnrB2, qnrB9, qnrB19, and bla(CMY-4) are described for the first time in Algeria and qnrB19 for the first time in non-clinical environments. This study highlights the major potential role of hospital effluents as providers of resistance genes to natural environments.

• 出版日期2014-4