Protein stabilization in a highly knotted protein polymer

作者：Sayre Tobias C; Lee Toni M; King Neil P; Yeates Todd O^*

来源：Protein Engineering Design and Selection, 2011, 24(8): 627-630.

DOI：10.1093/protein/gzr024

• biomaterials
• disulfide
• protein design
• protein knot
• topology

The polypeptide backbones of a few proteins are tied in a knot. The biophysical effects and potential biological roles of knots are not well understood. Here, we test the consequences of protein knotting by taking a monomeric protein, carbonic anhydrase II, whose native structure contains a shallow knot, and polymerizing it end-to-end to form a deeply and multiply knotted polymeric filament. Thermal stability experiments show that the polymer is stabilized against loss of structure and aggregation by the presence of deep knots.

• 出版日期2011-8