Dual High-Resolution alpha-Glucosidase and Radical Scavenging Profiling Combined with HPLC-HRMS-SPE-NMR for Identification of Minor and Major Constituents Directly from the Crude Extract of Pueraria lobata

作者：Liu Bingrui; Kongstad Kenneth T; Sun Qinglei; Nyberg Nils T; Jager Anna K; Staerk Dan^*

来源：Journal of Natural Products, 2015, 78(2): 294-300.

DOI：10.1021/np5009416

The crude methanol extract of Pueraria lobata was investigated by dual high-resolution a-glucosidase inhibition and radical scavenging profiling combined with hyphenated HPLC-HRMS-SPE-NMR. Direct analysis of the crude extract without preceding purification was facilitated by combining chromatograms from two analytical-scale HPLC separations of 120 and 600 mu g on-column, respectively. High-resolution alpha-glucosidase and radical scavenging profiles were obtained after microfractionation of the eluate in 96-well microplates. This allowed full bioactivity profiling of individual peaks in the HPLC chromatogram of the crude methanol extract. Subsequent HPLC-HRMS-SPE-NMR analysis allowed identification of 21 known compounds in addition to two new compounds, i.e., 3'-methoxydaidzein 8-C-[alpha-D-apiofuranosyl-(1 -> 6)]-beta-D-glucopyranoside and 6 ->-O-malonyl-3'-methoxydaidzin, as well as an unstable compound tentatively identified as 3'-de-O-methylpuerariafuran.

• 出版日期2015-2
• 单位test