Naked-eye quantitative aptamer-based assay on paper device

作者：Zhang, Yun^*; Gao, Dong; Fan, Jinlong; Nie, Jinfang^*; Le, Shangwang; Zhu, Wenyuan; Yang, Jiani; Li, Jianping

来源：Biosensors and Bioelectronics, 2016, 78: 538-546.

DOI：10.1016/j.bios.2015.12.003

• Aptamer
• Microfluidic paper-based analytical device
• Equipment-free quantitative detection
• Point-of-care testing
• Adenosine

This work initially describes the design of low-cost, naked-eye quantitative aptamer-based assays by using microfluidic paper-based analytical device (PAD). Two new detection motifs are proposed for quantitative mu PAD measurement without using external electronic readers, which depend on the length of colored region in a strip-like mu PAD and the number of colorless detection microzones in a multi-zone mu PAD. The length measuring method is based on selective color change of paper from colorless to blue black via formation of iodine-starch complex. The counting method is conducted on the basis of oxidation-reduction reaction between hydrogen peroxide and potassium permanganate. Their utility is well demonstrated with sensitive, specific detection of adenosine as a model analyte with the naked eye in buffer samples and undiluted human serum. These equipment-free quantitative methods proposed thus hold great potential for the development of more aptamer-based assays that are simple, cost-efficient, portable, and user-friendly for various point-of-care applications particularly in resource-constrained environments.

• 出版日期2016-4-15
• 单位桂林理工大学