Alleviation of osteoarthritis by calycosin-7-O-beta-D-glucopyranoside (CG) isolated from Astragali radix (AR) in rabbit osteoarthritis (OA) model

作者：Choi S I; Heo T R; Min B H; Cui J H; Choi B H; Park S R^*

来源：Osteoarthritis and Cartilage, 2007, 15(9): 1086-1092.

DOI：10.1016/j.joca.2007.02.015

• osteoarthritis
• Astragali radix
• isoflavonoid
• calycosin-7-o-beta-d-glucopyranoside

Objective: This work was undertaken to assess the protective effect of an isoflavonoid, calycosin-7-O-beta-D-glucopyranoside (CG), isolated from Astragali radix (AR) on the pathogenesis of osteoarthritis (OA)-like lesion in a rabbit model.
Methods: Nine rabbits underwent an anterior cruciate ligament and menisectomy transection (ACLMT) of the rear knee joints to induce OA-like lesion. They were randomly divided into three groups (n = 6/group): a negative control group treated with 200 mu l of 0.5% (v/v) dimethyl sulfoxide (DMSO), a positive control group treated with 200 VI of 100 mu M piroxicam, and a test group treated with 100 mu g/500 mu l of CG, where the test agents were administered by injection once a week for 4 weeks starting from the third week. Rabbits were then sacrificed to observe the progression of OA-like lesion. The synovial fluid was analyzed for the amounts of total proteins, glycosaminoglycans (GAG) and prostaglandin E-2 (PGE(2)). In addition, histopathologic analyses were performed on the OA-like articular cartilage with or without therapeutic treatments.
Results: The total synovial fluid volume (P < 0.05) was most strikingly reduced by the treatment with CG. Moreover, the CG treatment also significantly alleviated the OA-induced accumulation of prostaglandin (PG) (P < 0.001) and total proteins (P < 0.001) in the synovial fluid. The histopathologic analyses revealed that the CG treatment reduced the severity of the OA-like structural damages in the cartilage. However, the level of PGE2, a pathologic inflammatory molecule, was not diminished by CG or piroxicam.
Conclusion: These results indicate that the isoflavonoid CG isolated from AR significantly alleviated the pathologic changes in the OA-like rabbit knee joints. This suggests that CG from AR could be a promising treatment for the therapy of OA.

• 出版日期2007-9
• 单位河北医科大学