A holin remnant protein encoded by STY1365 is involved in envelope stability of Salmonella enterica serovar Typhi

作者：Rodas Paula I; Trombert A Nicole; Mora Guido C^*

来源：FEMS Microbiology Letters, 2011, 321(1): 58-66.

DOI：10.1111/j.1574-6968.2011.02310.x

• Salmonella
• holin
• inner membrane

We characterized STY1365, a small ORF of Salmonella enterica serovar Typhi. This 174-bp ORF encodes a putative product of 57 amino acid residues with a premature stop codon. Nevertheless, bioinformatic analyses revealed that the predicted product of STY1365 has similarity to putative holin genes of Escherichia coli and bacteriophage Phi P27. STY1365 showed a high-level expression at the early log phase and a small corresponding protein product was detected mainly in the inner membrane fraction. Cloning of STY1365 in pSU19 mid-copy-vector produced retardation in S. Typhi growth, increased cell permeability to crystal violet and altered the inner membrane protein profile. Similar results were obtained when STY1365 was induced with isopropyl-beta-D-thio-galactoside in pCC1 (TM) single-copy vector. Our results support the fact that S. Typhi STY1365 encodes a holin remnant protein that is involved in the stability of the bacterial envelope.

• 出版日期2011-8