NME2 associates with PTP sigma to transduce signals from chondroitin sulfate proteoglycans

作者：Hamasaki Hajime; Fujitani Masashi^*; Yamashita Toshihide^*

来源：Biochemical and Biophysical Research Communications, 2016, 471(4): 522-527.

DOI：10.1016/j.bbrc.2016.02.042

• CSPGs
• PTP sigma
• NME2
• Axon outgrowth

Chondroitin sulfate proteoglycans (CSPGs) are a major component of glial scars, inhibiting axonal growth in the central nervous system. Protein tyrosine phosphatase, receptor type S (PTP sigma) has been identified as a receptor for CSPGs, whereas its downstream signaling pathway remains to be fully understood. Here, we report that nucleoside diphosphate kinase 2 (NME2) interacts with PTP sigma. We screened proteins associated with PTP sigma by mass spectrometry, and obtained NME2. Immunoprecipitation analysis revealed that NME2 associated with the PTPa intracellular domain in HEK-293T cells. NME2 was expressed in the cytoplasm and nucleus of cortical neurons, and knockdown of NME2 in the cortical neurons completely rescued neurite outgrowth inhibition induced by CSPGs. These results demonstrate that NME2 associates with PTPa to elicit neurite outgrowth inhibition in response to CSPGs.

• 出版日期2016-3-18